Can 41 Targets Be Resolved in Just 6 Rounds of DNA-PAINT?
How combinatorial DNA barcoding resolves 41 targets in just 6 imaging rounds, breaking the linear scaling bottleneck in multiplexed super-resolution microscopy.
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Advanced Imaging & Multiplexing
11 articles • Revolutionary imaging techniques and applications
Revolutionary imaging techniques using DNA-barcoded conjugates, signal amplification methods, and high-resolution spatial analysis for advanced multiplexed protein detection.
Can Light Replace Wash Steps in DNA-PAINT?
How photocaged antibody-oligo conjugates eliminate buffer exchange in multiplexed DNA-PAINT, enabling light-controlled target switching for super-resolution microscopy.
Where Are the Enzymes, and Where Are They Active?
How activity-dependent proximity ligation (ADPL) combines activity-based chemical probes with antibody-oligo proximity ligation to detect active enzymes at single-cell and subcellular resolution.
Expanding Cells for Super-Resolution Imaging with AOC Signals Locked in Place
How DNA-PAINT docking strands can be covalently imprinted into hydrogel matrices during expansion microscopy, preserving nanoscale spatial information through embedding and clearing.
Immunofluorescence (IF) Protocol for Antibody-Oligo Conjugates
Complete immunofluorescence protocol for FFPE tissue staining using antibody-oligo conjugates. Step-by-step guide including antigen retrieval, blocking, staining with imaging strands, and troubleshooting.
Can DIY Microscopy Finally Democratize Spatial Biology?
Discover how Fluid-Squid combines open-source microscopy with automated fluidics for affordable 36-plex AOC imaging, making spatial proteomics accessible to all for around $30k.
Exploring the Next Frontier: Spatial Proximity Sequencing
Sprox-seq combines proximity ligation with spatial platforms to map protein-protein interactions in tissue. Profile 32 proteins and 528 pairwise interactions with preserved spatial coordinates.
AOC Techniques Continue to Advance: Solving Nuclear Imaging with Left-Handed DNA
Nuclear imaging presents unique challenges in spatial biology. Learn how left-handed DNA (L-DNA) oligonucleotide conjugates overcome nuclear membrane barriers for improved imaging.
Flexible Multiplexed Imaging via Cyclic Immunofluorescence with DNA-Barcoded Antibodies
Advances in spatial proteomics have underscored the need for highly multiplexed imaging platforms that can preserve tissue integrity while enabling comprehensive protein analysis.
Amplifying Discovery: How Antibody–Oligo Conjugates Power SABER-IMC
Imaging Mass Cytometry (IMC) has the potential to profile ~40 proteins in tissues but struggles to detect low-abundance markers. The latest advance—SABER-IMC (Signal Amplification By Exchange Reaction for IMC).
IN-DEPTH: IN-situ DEtailed Phenotyping To High Resolution
In the evolving field of biomarker discovery and spatial biology, IN-situ DEtailed Phenotyping To High Resolution (IN-DEPTH) represents a groundbreaking approach for high-dimensional cellular analysis.
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