Proximity Ligation Assay (PLA) Protocol
Complete PLA protocol for immunofluorescence format. Step-by-step guide including cell fixation, AOC binding, connector hybridization, ligation, rolling circle amplification, and troubleshooting tips.
2 articles • Applications in protein interaction studies
Applications in protein interaction studies and proximity detection using Proximity Ligation Assays (PLA) and related techniques for analyzing molecular interactions in biological systems.
Complete PLA protocol for immunofluorescence format. Step-by-step guide including cell fixation, AOC binding, connector hybridization, ligation, rolling circle amplification, and troubleshooting tips.
Introduction to Proximity Ligation Assays (PLA) Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly in cells and tissues.
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