8 articles • Protocols for single-cell protein detection
Protocols and optimization strategies for single-cell protein detection, staining optimization, and multi-omics workflows using antibody-oligonucleotide conjugates.
Antibody-oligonucleotide conjugate (AOC) and antibody-derived tag (ADT) describe the same reagent. AOC is the conjugation-chemistry, PLA and spatial term; ADT is the single-cell CITE-seq term. A guide to using and searching both, plus adjacent terms like HTO.
How antibody-DNA conjugates turn digital droplet PCR into a multiplexed single-EV protein assay, reaching a 38 EV/µL limit of detection and resolving PD-L1 heterogeneity across tumor-derived extracellular vesicles.
How NULISA engineers paired antibody-oligonucleotide conjugates to both purify intact immunocomplexes and generate a ligated reporter, reaching attomolar single-plex sensitivity and 200-plex multiplexing in NULISAseq.
Explore nELISA from Nomic Bio — a 191-plex immunoassay platform using DNA-mediated CLAMP technology that overcomes traditional ELISA multiplexing limitations with sub-picogram sensitivity.
Exploring the potential and challenges of universal antibody barcoding with oligonucleotides for research applications, and the implications for reproducibility and data sharing.
Protocols and optimization strategies for single-cell protein detection, including staining conditions, buffer optimization, and workflow considerations for multiomics applications.
Optimization of labeling ratios for enhanced performance in single-cell applications. Finding the optimal balance between signal strength and specificity in multi-omics workflows.
Survey of multiplexed techniques and applications enabled by AOCs, from proximity ligation assays to advanced imaging methods and single-cell analysis.
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