A literature analysis of 10,000+ open-access publications spanning 2001–2026. Maps who is leading PLA research, what they use it for (cancer, neurodegeneration, cell biology, signalling, immunology), the methods variants in use, geographic distribution, and the technique's lineage from Landegren's original 2002 invention through to in-situ sequencing.
Proximity Ligation Assay (PLA) is a highly sensitive molecular biology technique designed to detect and visualize protein-protein interactions directly in cells and tissues.
Complete Proximity Ligation Assay (PLA) protocol for immunofluorescence format. Step-by-step guide including cell fixation, AOC binding, ligation, rolling circle amplification, and troubleshooting.
How activity-dependent proximity ligation (ADPL) combines activity-based chemical probes with antibody-oligo proximity ligation to detect active enzymes at single-cell and subcellular resolution.
Sprox-seq combines proximity ligation with spatial platforms to map protein-protein interactions in tissue. Profile 32 proteins and 528 pairwise interactions with preserved spatial coordinates.
Tags that appear alongside “PLA” in articles.