CITE-seq

3 articles

Optimize CITE-seq experiments with antibody-oligo conjugates — practical guidance on staining parameters, degree of labeling, and data quality.

AOC ADT CITE-seq Terminology

Single-Cell & Multiomics

AOC vs ADT: Two Names for the Same Reagent

Antibody-oligonucleotide conjugate (AOC) and antibody-derived tag (ADT) describe the same reagent. AOC is the conjugation-chemistry, PLA and spatial term; ADT is the single-cell CITE-seq term, and the name of the protein readout in Seurat, dsb and ADTnorm. A guide to using and searching both.

14 Jul 2026Read article

CITE-seq Optimization Staining

Single-Cell & Multiomics

Optimizing Antibody Oligo-Conjugates Staining for Single-Cell Multiomics

In the fast-evolving landscape of multimodal single-cell analysis, antibody oligo-conjugates (AOCs) have revolutionized our ability to simultaneously profile gene expression and surface protein markers.

11 Feb 2025Read article

DoL CITE-seq Optimization

Single-Cell & Multiomics

Degree of Labeling Impacts ADTs for Single-Cell Multi-Omics

How the number of oligos per antibody (degree of labeling) shapes ADT signal-to-noise, specificity, and detection of low-expressing proteins in CITE-seq.

25 Mar 2025Read article

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Optimization3 AOC5 ADT1 Terminology1 Staining1 DoL2